phospho smad1 Search Results


smad1  (MedChemExpress)
92
MedChemExpress smad1
Phosphorylation of <t>Smad1/5/9</t> during VICs osteogenic differentiation. A. Cells were stimulated with IP-OIM for 15, 30, 60, and 120 min to detect Smad1/5/9 phosphorylation levels. B. The inhibitory efficiency <t>of</t> <t>the</t> <t>Smad1/5/9-specific</t> inhibitor Compound C (CC) was determined by pre-treating VICs with varying concentrations of Compound C. C. ALP activity was measured on the 6th day of co-culture with Compound C (N=3; *P<0.05, **P<0.001). D. ALP staining was performed on the 6th day. E. Detection of calcium content on the 9th day of Compound C treatment (N=3; *P<0.05, **P<0.001). F. Alizarin Red staining of cells on the 9th day. G. Phosphorylation levels of Smad1/5/9 in siRNA-transfected VICs stimulated with IP-OIM for 60 min.
Smad1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phospho smad1 5 rabbit monoclonal antibody antipmad  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc phospho smad1 5 rabbit monoclonal antibody antipmad
Phosphorylation of <t>Smad1/5/9</t> during VICs osteogenic differentiation. A. Cells were stimulated with IP-OIM for 15, 30, 60, and 120 min to detect Smad1/5/9 phosphorylation levels. B. The inhibitory efficiency <t>of</t> <t>the</t> <t>Smad1/5/9-specific</t> inhibitor Compound C (CC) was determined by pre-treating VICs with varying concentrations of Compound C. C. ALP activity was measured on the 6th day of co-culture with Compound C (N=3; *P<0.05, **P<0.001). D. ALP staining was performed on the 6th day. E. Detection of calcium content on the 9th day of Compound C treatment (N=3; *P<0.05, **P<0.001). F. Alizarin Red staining of cells on the 9th day. G. Phosphorylation levels of Smad1/5/9 in siRNA-transfected VICs stimulated with IP-OIM for 60 min.
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rabbit anti phospho smad1 5 9  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc rabbit anti phospho smad1 5 9
Phosphorylation of <t>Smad1/5/9</t> during VICs osteogenic differentiation. A. Cells were stimulated with IP-OIM for 15, 30, 60, and 120 min to detect Smad1/5/9 phosphorylation levels. B. The inhibitory efficiency <t>of</t> <t>the</t> <t>Smad1/5/9-specific</t> inhibitor Compound C (CC) was determined by pre-treating VICs with varying concentrations of Compound C. C. ALP activity was measured on the 6th day of co-culture with Compound C (N=3; *P<0.05, **P<0.001). D. ALP staining was performed on the 6th day. E. Detection of calcium content on the 9th day of Compound C treatment (N=3; *P<0.05, **P<0.001). F. Alizarin Red staining of cells on the 9th day. G. Phosphorylation levels of Smad1/5/9 in siRNA-transfected VICs stimulated with IP-OIM for 60 min.
Rabbit Anti Phospho Smad1 5 9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti phospho smad 1 5  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc anti phospho smad 1 5
Phosphorylation of <t>Smad1/5/9</t> during VICs osteogenic differentiation. A. Cells were stimulated with IP-OIM for 15, 30, 60, and 120 min to detect Smad1/5/9 phosphorylation levels. B. The inhibitory efficiency <t>of</t> <t>the</t> <t>Smad1/5/9-specific</t> inhibitor Compound C (CC) was determined by pre-treating VICs with varying concentrations of Compound C. C. ALP activity was measured on the 6th day of co-culture with Compound C (N=3; *P<0.05, **P<0.001). D. ALP staining was performed on the 6th day. E. Detection of calcium content on the 9th day of Compound C treatment (N=3; *P<0.05, **P<0.001). F. Alizarin Red staining of cells on the 9th day. G. Phosphorylation levels of Smad1/5/9 in siRNA-transfected VICs stimulated with IP-OIM for 60 min.
Anti Phospho Smad 1 5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p smad1  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc p smad1
Phosphorylation of <t>Smad1/5/9</t> during VICs osteogenic differentiation. A. Cells were stimulated with IP-OIM for 15, 30, 60, and 120 min to detect Smad1/5/9 phosphorylation levels. B. The inhibitory efficiency <t>of</t> <t>the</t> <t>Smad1/5/9-specific</t> inhibitor Compound C (CC) was determined by pre-treating VICs with varying concentrations of Compound C. C. ALP activity was measured on the 6th day of co-culture with Compound C (N=3; *P<0.05, **P<0.001). D. ALP staining was performed on the 6th day. E. Detection of calcium content on the 9th day of Compound C treatment (N=3; *P<0.05, **P<0.001). F. Alizarin Red staining of cells on the 9th day. G. Phosphorylation levels of Smad1/5/9 in siRNA-transfected VICs stimulated with IP-OIM for 60 min.
P Smad1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phospho smad1 5 ser463 465 41d10 rabbit mab  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc phospho smad1 5 ser463 465 41d10 rabbit mab
Phosphorylation of <t>Smad1/5/9</t> during VICs osteogenic differentiation. A. Cells were stimulated with IP-OIM for 15, 30, 60, and 120 min to detect Smad1/5/9 phosphorylation levels. B. The inhibitory efficiency <t>of</t> <t>the</t> <t>Smad1/5/9-specific</t> inhibitor Compound C (CC) was determined by pre-treating VICs with varying concentrations of Compound C. C. ALP activity was measured on the 6th day of co-culture with Compound C (N=3; *P<0.05, **P<0.001). D. ALP staining was performed on the 6th day. E. Detection of calcium content on the 9th day of Compound C treatment (N=3; *P<0.05, **P<0.001). F. Alizarin Red staining of cells on the 9th day. G. Phosphorylation levels of Smad1/5/9 in siRNA-transfected VICs stimulated with IP-OIM for 60 min.
Phospho Smad1 5 Ser463 465 41d10 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti phospho smad1 ser206  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc rabbit anti phospho smad1 ser206
Phosphorylation of <t>Smad1/5/9</t> during VICs osteogenic differentiation. A. Cells were stimulated with IP-OIM for 15, 30, 60, and 120 min to detect Smad1/5/9 phosphorylation levels. B. The inhibitory efficiency <t>of</t> <t>the</t> <t>Smad1/5/9-specific</t> inhibitor Compound C (CC) was determined by pre-treating VICs with varying concentrations of Compound C. C. ALP activity was measured on the 6th day of co-culture with Compound C (N=3; *P<0.05, **P<0.001). D. ALP staining was performed on the 6th day. E. Detection of calcium content on the 9th day of Compound C treatment (N=3; *P<0.05, **P<0.001). F. Alizarin Red staining of cells on the 9th day. G. Phosphorylation levels of Smad1/5/9 in siRNA-transfected VICs stimulated with IP-OIM for 60 min.
Rabbit Anti Phospho Smad1 Ser206, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit monoclonal anti psmad1 5 9  (Cell Signaling Technology Inc)
91
Cell Signaling Technology Inc rabbit monoclonal anti psmad1 5 9
Phosphorylation of <t>Smad1/5/9</t> during VICs osteogenic differentiation. A. Cells were stimulated with IP-OIM for 15, 30, 60, and 120 min to detect Smad1/5/9 phosphorylation levels. B. The inhibitory efficiency <t>of</t> <t>the</t> <t>Smad1/5/9-specific</t> inhibitor Compound C (CC) was determined by pre-treating VICs with varying concentrations of Compound C. C. ALP activity was measured on the 6th day of co-culture with Compound C (N=3; *P<0.05, **P<0.001). D. ALP staining was performed on the 6th day. E. Detection of calcium content on the 9th day of Compound C treatment (N=3; *P<0.05, **P<0.001). F. Alizarin Red staining of cells on the 9th day. G. Phosphorylation levels of Smad1/5/9 in siRNA-transfected VICs stimulated with IP-OIM for 60 min.
Rabbit Monoclonal Anti Psmad1 5 9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p ser 206 smad1 antibody  (Rockland Immunochemicals)
85
Rockland Immunochemicals p ser 206 smad1 antibody
Phosphorylation of <t>Smad1/5/9</t> during VICs osteogenic differentiation. A. Cells were stimulated with IP-OIM for 15, 30, 60, and 120 min to detect Smad1/5/9 phosphorylation levels. B. The inhibitory efficiency <t>of</t> <t>the</t> <t>Smad1/5/9-specific</t> inhibitor Compound C (CC) was determined by pre-treating VICs with varying concentrations of Compound C. C. ALP activity was measured on the 6th day of co-culture with Compound C (N=3; *P<0.05, **P<0.001). D. ALP staining was performed on the 6th day. E. Detection of calcium content on the 9th day of Compound C treatment (N=3; *P<0.05, **P<0.001). F. Alizarin Red staining of cells on the 9th day. G. Phosphorylation levels of Smad1/5/9 in siRNA-transfected VICs stimulated with IP-OIM for 60 min.
P Ser 206 Smad1 Antibody, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p smad1 5 9  (ABclonal Biotechnology)
94
ABclonal Biotechnology p smad1 5 9
Cytokines Activated Their Downstream Pathways in HK-2 Cells. The phosphorylation of ERK, JNK, p38, and NF-κB in HK-2 cells treated with TNF-α (A) or IL-1β (B) was analyzed over a time course ranging from 15 min to 4 h. The <t>phosphorylation</t> <t>of</t> <t>Smad1/5/9</t> and Smad2/3 in HK-2 cells treated with TGF-β1 (C) or BMP-2 (D) was analyzed over a time course ranging from 15 min to 4 h. GAPDH was served as the loading control.
P Smad1 5 9, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-smad 1/5/9  (ZenBio)
90
ZenBio anti-smad 1/5/9
Cytokines Activated Their Downstream Pathways in HK-2 Cells. The phosphorylation of ERK, JNK, p38, and NF-κB in HK-2 cells treated with TNF-α (A) or IL-1β (B) was analyzed over a time course ranging from 15 min to 4 h. The <t>phosphorylation</t> <t>of</t> <t>Smad1/5/9</t> and Smad2/3 in HK-2 cells treated with TGF-β1 (C) or BMP-2 (D) was analyzed over a time course ranging from 15 min to 4 h. GAPDH was served as the loading control.
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pe-labeled anti-smad1 (ps436/ps465)/smad8 (ps465/ps467  (Becton Dickinson)
90
Becton Dickinson pe-labeled anti-smad1 (ps436/ps465)/smad8 (ps465/ps467
Cytokines Activated Their Downstream Pathways in HK-2 Cells. The phosphorylation of ERK, JNK, p38, and NF-κB in HK-2 cells treated with TNF-α (A) or IL-1β (B) was analyzed over a time course ranging from 15 min to 4 h. The <t>phosphorylation</t> <t>of</t> <t>Smad1/5/9</t> and Smad2/3 in HK-2 cells treated with TGF-β1 (C) or BMP-2 (D) was analyzed over a time course ranging from 15 min to 4 h. GAPDH was served as the loading control.
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Image Search Results


Phosphorylation of Smad1/5/9 during VICs osteogenic differentiation. A. Cells were stimulated with IP-OIM for 15, 30, 60, and 120 min to detect Smad1/5/9 phosphorylation levels. B. The inhibitory efficiency of the Smad1/5/9-specific inhibitor Compound C (CC) was determined by pre-treating VICs with varying concentrations of Compound C. C. ALP activity was measured on the 6th day of co-culture with Compound C (N=3; *P<0.05, **P<0.001). D. ALP staining was performed on the 6th day. E. Detection of calcium content on the 9th day of Compound C treatment (N=3; *P<0.05, **P<0.001). F. Alizarin Red staining of cells on the 9th day. G. Phosphorylation levels of Smad1/5/9 in siRNA-transfected VICs stimulated with IP-OIM for 60 min.

Journal: American Journal of Translational Research

Article Title: Inorganic phosphate-osteogenic induction medium promotes osteogenic differentiation of valvular interstitial cells via the BMP-2/Smad1/5/9 and RhoA/ROCK-1 signaling pathways

doi:

Figure Lengend Snippet: Phosphorylation of Smad1/5/9 during VICs osteogenic differentiation. A. Cells were stimulated with IP-OIM for 15, 30, 60, and 120 min to detect Smad1/5/9 phosphorylation levels. B. The inhibitory efficiency of the Smad1/5/9-specific inhibitor Compound C (CC) was determined by pre-treating VICs with varying concentrations of Compound C. C. ALP activity was measured on the 6th day of co-culture with Compound C (N=3; *P<0.05, **P<0.001). D. ALP staining was performed on the 6th day. E. Detection of calcium content on the 9th day of Compound C treatment (N=3; *P<0.05, **P<0.001). F. Alizarin Red staining of cells on the 9th day. G. Phosphorylation levels of Smad1/5/9 in siRNA-transfected VICs stimulated with IP-OIM for 60 min.

Article Snippet: Subsequently, three working doses of the Smad1/5/9-specific inhibitor Compound C (MCE, New Jersey, USA) were added into the complete medium, and the VICs were pre-treated with these for 1 day.

Techniques: Phospho-proteomics, Activity Assay, Co-Culture Assay, Staining, Transfection

Cytokines Activated Their Downstream Pathways in HK-2 Cells. The phosphorylation of ERK, JNK, p38, and NF-κB in HK-2 cells treated with TNF-α (A) or IL-1β (B) was analyzed over a time course ranging from 15 min to 4 h. The phosphorylation of Smad1/5/9 and Smad2/3 in HK-2 cells treated with TGF-β1 (C) or BMP-2 (D) was analyzed over a time course ranging from 15 min to 4 h. GAPDH was served as the loading control.

Journal: Frontiers in Cell and Developmental Biology

Article Title: Inflammation-induced osteogenic signaling promotes calcium phosphate crystal formation in kidneys via MAPK, NF-κB, and smad pathways

doi: 10.3389/fcell.2026.1831072

Figure Lengend Snippet: Cytokines Activated Their Downstream Pathways in HK-2 Cells. The phosphorylation of ERK, JNK, p38, and NF-κB in HK-2 cells treated with TNF-α (A) or IL-1β (B) was analyzed over a time course ranging from 15 min to 4 h. The phosphorylation of Smad1/5/9 and Smad2/3 in HK-2 cells treated with TGF-β1 (C) or BMP-2 (D) was analyzed over a time course ranging from 15 min to 4 h. GAPDH was served as the loading control.

Article Snippet: Primary antibodies for immunoblotting such as OPN (A19092), ALP (A0514), OPG (A2100), Runx2 (A2851), p-JNK1/2/3 (AP0631), JNK1/2/3 (A4867), p-NF-κB (AP0944), NF-κB (A19653), p-p38 (AP0057), p38 (A14401), p-ERK (AP0485), ERK (A4782), p-Smad1/5/9 (AP0850), Smad1 (A21734), p-Smad2/3 (AP0548), Smad2/3 (A7536), TNF-α (A11534), IL-1β (A11534), TGF-β1 (A16640), BMP-2 (A0231), β-actin (AC028), α-Tubulin (A6830), and GAPDH (A19056) were from ABclonal (Woburn, MA, United States).

Techniques: Phospho-proteomics, Control